4^th FeSBioNet Training School 

COST FeSBioNet training school – Calorimetry for metalloprotein analysis and enzymology

Dates: June 24-25 2019
Location: Department of Biotechnology, Delft University of Technology, Van der Maasweg 9, 2629HZ, The Netherlands
Registration: https://goo.gl/forms/kEcHJOBWnEOkfSRm2
Training Grants are available for FeSBionet participants

Lecturers:

• Dr. P.L. (Peter-Leon) Hagedoorn (Delft University of Technology, NL)
• Dr. K.H. (Kourosh) Ebrahimi (Oxford University, UK)

Assistants:

• Marc Strampraad
• Dr. Tania Shams
• Dr. Jie Zhang

Virtually all physical, chemical or biological processes are associated with heat effects. Quantitative measurement of the heat evolution over time can be used to monitor the progression and rate of these processes. Isothermal Titration Calorimetry has been successfully applied to measure binding of metal ions and molecules to proteins and binding interactions of proteins involved in iron-sulfur cluster biosynthesis, in order to determine thermodynamic parameters and characterize different binding sites and stoichiometry. The same technique can be applied to measure enzyme kinetics and kinetics of cofactor transfer, without the need to measure substrate/product levels, but instead measure the heat that is being developed (or consumed) during a chemical reaction.
In order to perform ITC measurements to study iron-sulfur cluster biosynthesis processes anaerobic conditions are required. Furthermore there are considerable precautions that need to be taken in order to obtain accurate and useful binding and kinetic parameters. This training schools offers introduction, theory and best practices to design and perform ITC experiments for the analysis of metalloproteins and enzymes.

Training School Program

Monday June 24, 2019
9:00hr arrive
9:30-9:10:15hr lecture – biocalorimetry principles and concepts
10.30-11:15hr lecture – isothermal titration calorimetry to measure metal ion binding to proteins
11:30-12:15hr exercises – design experiments for the afternoon practical – participants can bring their own samples and cases
12:15-13:30hr lunch
13:30-17:00hr lab exercise – ITC / data analysis exercises
17:30hr closing day 1
18:00hr small tour Delft
19:00hr dinner downtown Delft
21:00hr drinks or free time

Tuesday June 25, 2019
9:00-9:45hr recap day 1 and lecture – enzyme kinetics
10:00-10:45hr lecture – enzyme calorimetry
11:00-12:15hr lab exercise – enzyme calorimetry – (ITC/microfluidic calorimeter) / data analysis exercises
12:15-13:30hr lunch
13:30-15:30hr lab exercise – enzyme calorimetry – (ITC/ microfluidic calorimeter) / data analysis exercises
15:30-17:00hr Results and summary
17:00hr end (travel back or stay for dinner)

Getting there

Airplane
Schiphol International Airport is one of the central airports. All the well-known airlines are flying from and to Schiphol on a daily base. Transportation from Schiphol Airport to Delft is possible by public transport or the special taxi service called Schiphol Travel Taxi. There is a direct train connection between Schiphol International Airport and Delft railway station (plan your train journey). Travelling time is about 40 minutes; the frequency is 5 times per hour.
Apart from Schiphol International Airport there is the smaller and more Western European orientated Rotterdam The Hague Airport. The airport is approximately a 15-minute drive from the centre of Delft.

Train, tram and buses
From the ‘Delft Centraal’ train station you can see the towers of the Old Church and walk to the city centre in only a few minutes. It only takes 10 minutes to get from Rotterdam and/or The Hague to Delft by train, 45 minutes from Amsterdam. Tram 1 will take you to The Hague in about 30 minutes and to Scheveningen beach in about 45 minutes.
If you are coming by public transport, take the train or bus to Delft Central Station. The following buses travel between Delft Central Station and building 58:
Bus 40: get off at bus stop "TU - Kluyverpark, Delft"
Bus 69: get off at bus stop "TU - Kluyverpark, Delft"
Bus 174: get off at bus stop "TU - Kluyverpark, Delft"

See Journey planner 9292: https://9292.nl/
More information: https://www.tudelft.nl/en/about-tu-delft/contact-and-accessibility/map-and-buildings/building-58/